I'm looking for cells could express human IL4 receptor by itself instead of foreign gene transformed. I test the IL4R on Daudi, but less expression.
Hi, I want to estblish a drug resistant cancer cell line, Dose anyone could give a hand about it? is there some protocols or refferences/books? PS: If I want to treat the cell with drug, what...
11 December 2018 2,798 0 View
I got a fragment of DNA from the mouse feces by PCR. while there was no results while I blast in the NCBI,what should I do next?
31 December 2016 4,347 3 View
How to evaluate the propagation of DNA virus? what's methods can be used? Quantitive-PCR to detect DNA of the cell culture at different time post infection and RT-PCR to detect the RNAs about...
04 May 2016 9,043 1 View
Invasion of virus into the cell is by the receptor and ligand.In what way to verify the receptor?
31 December 2014 2,507 6 View
How can we to improve the titer of the virus in the cell culture?A strain of porcine parvovirus was isolated from a diseased pig. and it was cultivted for 3month and 30 passages,The TCID50 of the...
31 December 2014 6,936 9 View
It is isolated from a sick pig. We cultured it on blood agar and chocolate agar, which the colony shape like a wax drops(see picture). The16srRNA sequence analyses indicate that it is...
09 October 2014 8,637 4 View
Actually, I'm doing some work about the chitosan. Chitosan has been used in many fields, food, medicine, etc. It is also used in adjuvant for mucosal immunity with antigen. Anyone has similar work...
09 October 2013 3,399 3 View
I've got a humanized bi-specific monoclonal antibody targeting to PD-1 and CTL-4. how can i verify the function of it? what experiment should be taken?
01 January 1970 1,761 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View