How to establish drug resistant cancer cell lines?

Hi, I want to estblish a drug resistant cancer cell line, Dose anyone could give a hand about it? is there some protocols or refferences/books? PS: If I want to treat the cell with drug, what...

11 December 2018 2,678 0 View

How canI verify the DNA fragment without results after blast in NCBI?

31 December 2016 4,235 3 View

The method to estimate the virus propagation ?

How to evaluate the propagation of DNA virus? what's methods can be used? Quantitive-PCR to detect DNA of the cell culture at different time post infection and RT-PCR to detect the RNAs about...

04 May 2016 8,947 1 View

What way can we improve the titer of the virus during the cell culture?

31 December 2014 6,841 9 View

How can I verify the receptor of a virus?

Invasion of virus into the cell is by the receptor and ligand.In what way to verify the receptor?

31 December 2014 2,331 6 View

Dose anyone know what type of "bacterium" this is?

It is isolated from a sick pig. We cultured it on blood agar and chocolate agar, which the colony shape like a wax drops(see picture). The16srRNA sequence analyses indicate that it is...

09 October 2014 8,534 4 View

Does anyone work on adjuvant, especially chitosan?

Actually, I'm doing some work about the chitosan. Chitosan has been used in many fields, food, medicine, etc. It is also used in adjuvant for mucosal immunity with antigen. Anyone has similar work...

09 October 2013 3,306 3 View

How do i verify the function of bi-specific antibody?

01 January 1970 1,667 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

No title

28 February 2021 9,936 2 View