How to establish drug resistant cancer cell lines?

Hi, I want to estblish a drug resistant cancer cell line, Dose anyone could give a hand about it? is there some protocols or refferences/books? PS: If I want to treat the cell with drug, what...

11 December 2018 2,678 0 View

Does anyone give me some tips of which cell lines express hIL4 receptor?

I'm looking for cells could express human IL4 receptor by itself instead of foreign gene transformed. I test the IL4R on Daudi, but less expression.

03 April 2018 9,225 0 View

The method to estimate the virus propagation ?

How to evaluate the propagation of DNA virus? what's methods can be used? Quantitive-PCR to detect DNA of the cell culture at different time post infection and RT-PCR to detect the RNAs about...

04 May 2016 8,947 1 View

What way can we improve the titer of the virus during the cell culture?

31 December 2014 6,841 9 View

How can I verify the receptor of a virus?

Invasion of virus into the cell is by the receptor and ligand.In what way to verify the receptor?

31 December 2014 2,331 6 View

Dose anyone know what type of "bacterium" this is?

It is isolated from a sick pig. We cultured it on blood agar and chocolate agar, which the colony shape like a wax drops(see picture). The16srRNA sequence analyses indicate that it is...

09 October 2014 8,534 4 View

Does anyone work on adjuvant, especially chitosan?

Actually, I'm doing some work about the chitosan. Chitosan has been used in many fields, food, medicine, etc. It is also used in adjuvant for mucosal immunity with antigen. Anyone has similar work...

09 October 2013 3,306 3 View

How do i verify the function of bi-specific antibody?

01 January 1970 1,667 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View