How to establish drug resistant cancer cell lines?

Hi, I want to estblish a drug resistant cancer cell line, Dose anyone could give a hand about it? is there some protocols or refferences/books? PS: If I want to treat the cell with drug, what...

11 December 2018 2,678 0 View

Does anyone give me some tips of which cell lines express hIL4 receptor?

I'm looking for cells could express human IL4 receptor by itself instead of foreign gene transformed. I test the IL4R on Daudi, but less expression.

03 April 2018 9,225 0 View

How canI verify the DNA fragment without results after blast in NCBI?

31 December 2016 4,235 3 View

The method to estimate the virus propagation ?

How to evaluate the propagation of DNA virus? what's methods can be used? Quantitive-PCR to detect DNA of the cell culture at different time post infection and RT-PCR to detect the RNAs about...

04 May 2016 8,947 1 View

What way can we improve the titer of the virus during the cell culture?

31 December 2014 6,841 9 View

How can I verify the receptor of a virus?

Invasion of virus into the cell is by the receptor and ligand.In what way to verify the receptor?

31 December 2014 2,331 6 View

Does anyone work on adjuvant, especially chitosan?

Actually, I'm doing some work about the chitosan. Chitosan has been used in many fields, food, medicine, etc. It is also used in adjuvant for mucosal immunity with antigen. Anyone has similar work...

09 October 2013 3,306 3 View

How do i verify the function of bi-specific antibody?

01 January 1970 1,667 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View