Can anyone explain the difference between the MALDI-TOF/TOF or MALDI-TOF/MS-MS and Edman Sequencing of protein. Which analytic method gives the exact amino acid sequence of the protein/peptide?
I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can...
03 April 2018 3,971 0 View
During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...
01 February 2017 5,846 12 View
Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...
02 March 2016 5,056 4 View
Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...
31 December 2015 4,880 3 View
What is the difference between gel extraction and PCR clean up???
11 December 2015 334 4 View
Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"
09 October 2015 346 3 View
I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.
06 July 2015 471 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View