I am in need of Protein Identification using MALDI-TOF. Can anyone suggest me some labs or instititue?

I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can...

03 April 2018 3,905 0 View

How to select the buffer system for pH studies?

During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...

01 February 2017 5,770 12 View

How can I calculate L-asparaginase enzyme unit?

Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...

02 March 2016 4,984 4 View

Can any one kindly suggest me some research labs or sequencing company which offers Phylogentic tree and Dendrogram construction services?

Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...

31 December 2015 4,826 3 View

PCR: Gel extraction Vs PCR clean up?

What is the difference between gel extraction and PCR clean up???

11 December 2015 225 4 View

Can anyone explain me, how to write a propoer research hypothesis?

Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"

09 October 2015 262 3 View

Is someone in Proteomic research? Do anyone know any expert in proteomic research?

I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.

06 July 2015 396 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

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23 February 2021 4,014 3 View