I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.
I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can...
03 April 2018 3,971 0 View
During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...
01 February 2017 5,846 12 View
Can anyone explain the difference between the MALDI-TOF/TOF or MALDI-TOF/MS-MS and Edman Sequencing of protein. Which analytic method gives the exact amino acid sequence of the protein/peptide?
11 December 2016 9,657 0 View
Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...
02 March 2016 5,056 4 View
Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...
31 December 2015 4,880 3 View
What is the difference between gel extraction and PCR clean up???
11 December 2015 334 4 View
Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"
09 October 2015 346 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this
05 August 2024 7,902 3 View
i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...
02 August 2024 2,420 3 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 2,627 1 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
I want to know that n-doped side of solar cell is connected with positive or negative electrode.
20 July 2024 1,348 2 View