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Questions related from Yogeswaran Jagadeesan
I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can...
04 April 2018 3,951 0 View
During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...
02 February 2017 5,815 12 View
Can anyone explain the difference between the MALDI-TOF/TOF or MALDI-TOF/MS-MS and Edman Sequencing of protein. Which analytic method gives the exact amino acid sequence of the protein/peptide?
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Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...
03 March 2016 5,027 4 View
Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...
01 January 2016 4,864 3 View
What is the difference between gel extraction and PCR clean up???
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Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"
10 October 2015 313 3 View
I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.
07 July 2015 447 0 View