I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can provide appreciable results with interpretation
During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...
01 February 2017 5,770 12 View
Can anyone explain the difference between the MALDI-TOF/TOF or MALDI-TOF/MS-MS and Edman Sequencing of protein. Which analytic method gives the exact amino acid sequence of the protein/peptide?
11 December 2016 9,559 0 View
Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...
02 March 2016 4,984 4 View
Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...
31 December 2015 4,826 3 View
What is the difference between gel extraction and PCR clean up???
11 December 2015 225 4 View
Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"
09 October 2015 262 3 View
I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.
06 July 2015 396 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View