I am in need of Protein Identification using MALDI-TOF. Can anyone suggest me some labs or instititue?

Yogeswaran Jagadeesan @Yogeswaran_Jagadeesan

04 April 2018 0 4K Report

I need to do protein sequencing or identification through MALDI-TOF or N-Terminal sequencing. Can anyone suggest me some labs are institution which outsourcing samples for sequencing those who can provide appreciable results with interpretation

Badges
Science topic

More Yogeswaran Jagadeesan's questions See All

How to select the buffer system for pH studies?

During the optimum pH studies of an enzyme, should we change the assay buffers according to the pH or can we change the pH of the buffer itself Example: For a given enzyme, the assay mixture...

01 February 2017 5,846 12 View

Does anyone explain the difference between MALDI-TOF/and Edman Sequencing?

Can anyone explain the difference between the MALDI-TOF/TOF or MALDI-TOF/MS-MS and Edman Sequencing of protein. Which analytic method gives the exact amino acid sequence of the protein/peptide?

11 December 2016 9,657 0 View

How can I calculate L-asparaginase enzyme unit?

Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...

02 March 2016 5,056 4 View

Can any one kindly suggest me some research labs or sequencing company which offers Phylogentic tree and Dendrogram construction services?

Hi All, I am in need of bioinformatic expertise help in my research. I need to show my results of nucleotide sequences of my therapeutic enzymes in form of phylogentic or dendrogram form. But,...

31 December 2015 4,880 3 View

PCR: Gel extraction Vs PCR clean up?

What is the difference between gel extraction and PCR clean up???

11 December 2015 334 4 View

Can anyone explain me, how to write a propoer research hypothesis?

Can anyone explain me, how to write a proper research hypothesis? Not by using conventional "If" and "Then"

09 October 2015 346 3 View

Is someone in Proteomic research? Do anyone know any expert in proteomic research?

I need to sequence my enzymes. In this connection I have few queries regarding the methodology adopted for the same.

06 July 2015 471 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Using OBD technique i am trying to measure laser induced shockwaves velocity i found that at start velocity increases and then decay?

i am unable to interpret why its increases in start as shown in figure

11 August 2024 2,179 1 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can I interpret the data without the need of solving it manually?

How can I interpret the data gathered without solving?

03 August 2024 9,054 3 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View