It doesnt look like the caveolin marker I am using is going to the membrane, does anyone have any suggestions?
Dear Colleagues, I have started growing SLICE Culture in our laboratory. I dont have any reporter gene cloned, I would like to ask, how do I make sure that the culture is growing and the cells in...
02 March 2019 8,572 2 View
So far I see people do Co culture to study in vitro myelination assay. It will be helpful for me if we can keep the cells separately and still do the assay. Thanks a lot.
31 December 2018 3,603 2 View
We are working on virapower with plenti6 from invitrogen from company manual they say its really one of the safest formats of lenti viral mediated gene delivery. Do we have any published document...
01 February 2018 5,502 1 View
11 December 2016 2,711 1 View
Dear researchers, I am facing problem in maturating my OPCs into mature oligodendrocytes.. I have been using T3 and T4, also adding NAC and CNTF in the maturation media, even after 2 weeks I dont...
09 October 2016 6,786 2 View
I have started isolating and culturing primary glial cells from pig. So far it has been working fine, but After certain passage of OPC my cells, as it looks like, not responding to differentiating...
06 July 2016 4,615 0 View
I like to establish primary cultures (mainly neurons, oligodendrocytes and astrocytes) from brain of higher organisms.. I like to ask the experienced people what is the easiest way and simplest...
02 March 2016 4,454 2 View
I am interested to start working on human oligodendrocytes. My colleagues had purchased it from ATCC and it turned out to be not good. Can anyone recommend me some good and authentic source of...
02 March 2015 1,962 1 View
If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...
08 September 2014 6,348 6 View
During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...
07 August 2014 7,418 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View