Does anybody provide me a simple protocol to develop drug resistant (5FU) cancer cell line?

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Go J Yoshida

Incubation was carried out continuously and fresh drug-containing medium was changed at 24-h intervals. The concentration of 5-FU depends on the cell context, so that you have to check the lethal concentration for your cells.

http://www.nature.com/bjc/journal/v96/n1/full/6603496a.html

Jose Schneider

Nothing to add to the really excellent article by Yoshida