Abaqus Error: The surface of a 3D discrete rigid part does not exist in the assembly?

I used a discrete rigid part (cuboid) to translate a force into a plate for a bending test. Trying to run the job, there occurs an error that the surface of the cuboid does not exist. In the...

20 January 2020 5,160 4 View

Auto Sampler 96 well plate Flow cytometer?

We are interested in opinions, comments and recommendations regarding the automated measurement of 96 well plates using Accuri C6plus, Attune NxT and Cytoflex flow cytometers. Any experiences and...

27 November 2018 4,688 2 View

Difference between isenthalpic and adiabatic?

Hello, when is an adiabatic thermodynamic process (dQ=0) also an isenthalpic (dH=0) process? Regards Lothar

12 November 2018 5,600 13 View

How to deal with student's evaluations?

In order to improve own's teaching it is essental to get constructive feedback, The only form this feedback is organized and instituionalized (at the moment at least) is student's evaluation with...

07 May 2018 2,321 28 View

Does anyone have citations of insects feeding on reed (Phragmites australis) in China?

I am looking for any citation not yet recorded in my database.

19 December 2017 4,210 3 View

Why is the impact factor of ALTEX not listed in papers published there?

Just wondering, because ATLA is listed...Although much lower

06 July 2016 1,004 0 View

A diabetes out patient clinic "on demand"?

29 June 2016 6,349 3 View

Strict syllabus or improvisation?

Following the "teaching the subject or teaching students" thread, I want to ask how strictly one should follow a syllabus in order to communicate with student's needs. It's easy to say: let's...

07 December 2015 7,793 65 View

Why do transformed TOP10 grow on amp plates and not in amp LB fluid?

Hello everybody, I am having issues in constructing/ cloning a plasmid in TOP10 E. coli cell lines. We started contructing with a pGEX plasmid and our inserts (length: appr. 250 bp and 350 bp)....

15 October 2014 2,993 16 View

What is a good device to measure blood pressure non invasively in isoflurane anesthetized C57Bl/6?

Hi everyone, I am doing in vivo imaging in the kidney of mice and I want to measure the blood pressure of the mice while I am imaging them. I want to control the anesthesia depth on one hand but...

29 September 2014 6,406 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View