Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

How to embed RGD into PCL membrane?

I am currently working on a project Organ-on-a-chip for my dissertation, and we are using PCL membranes to culture MCF12A breast cells. However, I wanted to add RGD to increase cell adherence to...

14 August 2018 6,823 2 View

mRNA Transfection with Superfect?

Hello at all, Inspired through a paper that used the SuperFect Transfection Reagent to transfect siRNA to A431 cells I purchased it myself with the aim to transfect eGFP mRNA to HEK 293 cells. I...

26 October 2017 404 3 View

Why does the probing for this antibody look so bad?

Hi all, lately I have been struggling a lot with getting a good signal for a specific antibody on my western blot. I am particularly worried cause this antibodies works well for other lab mates of...

03 November 2015 8,624 1 View

For how long should rat limb be kept in 4%PFA @4C for fixation prior to decalcification?

I need this for some fresh tissue samples I will be receiving soon, and have only done this on ApoE and MC4R mouse limbs, which we usually fix overnight (~24hours).

17 December 2014 8,574 3 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

[Covid-19] GGO Evaluation - CT imaging of the lungs ?

13 January 2021 3,115 4 View

Where can I find a dataset for kidney stones images (CT or ultrasound)?

I want to apply neural network on kidney stones images (whether its CT images or ultrasound) to determine whether the kidney has stones or not.

02 January 2021 9,938 3 View

How i can extract the dose delivered to each pixel of CT image of TPS?

I generated a treatment plane for a patient using intensity modulated radiation therapy (IMRT). I intend to calculate cell survival in each voxel of target volume using LQ model. So, i need to...

03 November 2020 6,132 3 View

Does anyone know where I could find CT Dicom files to download with Contoured area around tumor ?

I am looking for CT images of different Cancer cell like ( Brain- Head&Neck - Liver- Lung and Prostate ) with contoured area that shows me where is the tumor exactly. I need to Download Dicom...

19 October 2020 3,545 6 View

In simple terms: to what extent do you believe classicial inversion frameworks provide more "general" estimates than learned frameworks?

This is more of a survey question than a query for precise mathematical detailing. Opinions are welcome!

10 September 2020 6,516 2 View

Convolutional Neural network for stack of CT or MRI images?

Hello; I know how to train image classifiers (CNN) for classification of single cross-sectional CT or MRI image. However, I don't know how to send all images from one patient to the model. I need...

10 September 2020 9,157 4 View

QPCR for Knockout gene expression check?

Hi everyone, I am working on mice that having one of their genes knocked out I have to check and confirm the knock out by qPCR, so in qPCR, only the WT samples showed CT value while the KO samples...

18 August 2020 1,443 1 View

Clinical Trail: How can we justify statistically a CT when it fails at final stage?

When a clinical trail succeed in first trails and fails at final trail,please write me, how could we justify the cause behind its failure statistically.

10 August 2020 8,035 3 View

Where could I find3D resistivity/conductivity (ERT) opensource data?

Hi, I would like to know whether is there any open source 3D inversion resistivity/conductivity geophysical data (ERT) available. If yes, how to access it?

06 August 2020 8,209 3 View