From your experience, how a research question should be written in a paper of policy sociology so that the research result leads to policy change?

Phd. Student, I am in the stage where I outline my research questions and research methodology. Thank you in advance for your support! A clarification: the chosen theme is the process of social...

16 October 2019 2,775 25 View

Is the pancreatic tissue still sensitive to pharmacological treatment after freezing in O.C.T. ?

I collected the pancreas from rats and stored them in OCT at -80 degrees C. I intend to treat the tissue cuts by immunosuppressors like FK506 or rapamycin for IHC analysis. Thank you for your...

03 May 2014 7,497 2 View

Is it possible to do MLR (mixed lymphocyte reaction) with monocytes and allogeneic CD4+ T cells?

I want to check my stimulated monocytes ability to present antigen to CD4+ T cells. Also wondering if I analyze the result on FACS, do I need to irradiate the monocytes? Any tips and inputs are...

28 January 2014 9,144 4 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

What is the best way to express Kruskal-Wallis test result visually (through graph)?

I have conducted an experiment regarding anthracnose disease of strawberry and the data collected for disease index appears to be non-normal distribution. As such, I conducted Kruskal-Wallis test...

11 December 2019 5,184 1 View

What internal control for qPCR of fungi detection in plants is recommended ?

I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...

11 July 2019 7,756 2 View

Biology and geometry: pentagon shape in strawberry?

I have just observed on the number of strawberries I was able to eat today that: -when you carefully take the leaves off the strawberry -the back of the strawberry shows a pentagonal shape See...

05 June 2019 395 5 View

Articles or Books about Strawberry plant architecture?

03 April 2019 8,724 2 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 7,547 3 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 4,817 3 View

Why my HPLC baseline shifts up??even the standard samples?

I would like to measure the flavonoids of strawberries by HPLC. The solvent A is 0.01% TFA in water and the solvent B is 0.01% TFA in acetonitrile. The column is C18. The base line always go up...

08 September 2018 7,136 0 View

Anyone to recommend books and websites with a comprehensive layout of Scripting in Materials Studio with Perl language?

Molecular Dynamics Simulation Perl Strawberry Scripting

06 July 2018 6,041 2 View