Hi! Specifically for fluorescently-labeled beta-amyloids you may have a look at the Anaspec websites. Here in Europe they have different distributors, e.g. Mobitec.

Good luck with your project!

Thanks! fluorescent amyloids I found, problems start with tyhe rest of the proteins...

Although it is not selective, Thioflavin T (ThT) is always a good start:

www.assay-protocol.com/.../thioflavin-t-s...

Thanks, but the assay-protocol page does only return the established protocols and I could not find any reference to thioflavin there. Or as a matter of fact there are entries where thioflavin seves to detect the beta-amyloid fibril formation. What I need is a way to make a fluorescent conjugate of alpha-synuclein or tau...

Not sure what your goal is, it should be no difference for fluorescence labeling tau or any other polypeptide or poteins. I think the regular fluorescence labeling should work for tau or any other proteins:

http://pharmaxchange.info/press/2011/01/fluorescent-labeling-of-biomolecules-with-organic-probes/

Now THIS ONE is helpful, many thanks!

For tau you could kindly ask Marc Diamond.

HI!

I am still not sure what is your aim. But as far and I am concern,ThT has no fluorescence intensity by itself; however, when it binds to fibril form of protein it has fluorescence (Excitation 440, Emission 480). In fact it insert to the beta-sheet structures and intensity. Nevertheless you cannot ThT as it is in your experiment. You have to replace an amino acid in the sequence of whether alpha-synuclein, tau and beta-amyloid (no difference between them), by the specific amino acid which has the florescent intensity somehow it does not interfere in fibril formation or the protein structure and function e.g cysteine and then purified it and assess it by the specific fluorescence dye as we did in our experiment. There are lots of prediction program including AGGRESCAN, PASTA, TANGO which you can use for such changes. Now we have the alpha-synuclein with we are able to label it by the specific florescence dye and evaluate it in a different aspect of our work. The manuscript has been ready and soon will be published.

Hi, luminescent conjugated oligothiophenes may help. They detect amyloid beta, synuclein and tau fibrils. you may check my publication and/or “Luminescent conjugated oligothiophenes: optical dyes for revealing pathological hallmarks of protein misfolding diseases.” by  “Per Hammarström, Mikael Lindgren, K. Peter R. Nilsson” and the follow up studies. In case you want to use in vivo, intravenous application of them works good and easy. The dyes spectral characteristics depend on what they bind to (tau, amyloid beta etc)

good luck!

Keep in mind that the specific construct of tau that you use with the fluorescent tag will modify its ability to aggregate. Our lab (Marc Diamond's) has had less luck with 4R2N wildtype fibril aggregation, but better luck with 4R2N P301S or L aggregation. The repeat domain with a fluorescent tag is amenable to seeded aggregation in general, unless you modify the hexapeptide motifs that induce aggregation. If you are looking simply for tau that has a fluorescent tag but that does not need to aggregate, any of these will work.

Hi Jacek,

I'm not sure if you're still looking into this, but we offer a variety of alpha-synuclein and tau fibrils, and can make custom fluorescent conjugates: www.stressmarq.com/PFFs