Gonca Bayraktar

2 Questions 5 Answers 0 Followers

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How stably Renilla Luc. is expressed among samples in a dual luciferase assay experiment?

Hi all, I am wondering if everyone gets a stable expression hence readout of Renilla Luciferase among different samples, which is used as an internal control for the normalisation of Firefly...

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How do I judge the HKG variation among samples in qRTPCR?

Hi, I would like to ask how much of variation in the Ct values of the HKG among experiment and control samples is accepted. For examples HPRT gene Ct values for the control group varies from 31 to...

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