Does anybody know another form of generate superoxide radicals for superoxide dismutase determination diferent of xanthine and xanthine oxidase?

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Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Are the coordinates the center of the cell or a corner in GADM / worldclim maps in R?

I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...

15 July 2024 8,844 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

What is your preference regarding Artificial Intelligence apps/methods/platforms for image analysis?

Please, let me know the apps, platforms, or methodologies based on AI to analyze images, such as radiographic or histology images. Tell me your experience of using AI in assessing patterns,...

30 June 2024 4,430 4 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

Why are my nanoliposomes turning purple??

Hello, I am making lipid nanoparticles using the thin film hydration method. Formula is DODMA:DSPC:Cholesterol:DSG-PEG2000 at 60:20:17.5:2.5 %mM in 5mM total. After hydration in sodium acetate...

16 June 2024 6,842 6 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

¿Cuánto conoce usted sobre sistemas de humidificación para pacientes en ventilación mecánica?

A través de este link que figura a continuación lo invitamos a participar del estudio "Encuesta de Enfoque Doble con Énfasis en Sistemas de Humidificación de Calentado...

09 June 2024 8,350 0 View

What skills should today's teachers take into account to teach students and prepare them for a long-term future?

Taking into account that each society incorporates into the curricula the necessary knowledge to teach depending on the social demands of each country.

06 June 2024 9,710 14 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Does anyone have this article in PDF and could they pass it on to me?

Paper: Wang, A.G., Luo, G.H., 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 6, 55–57.

19 June 2024 7,283 0 View

What should I use to homogenize samples for measurements of Oxidative Stress?

I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...

07 May 2024 4,583 2 View

Incubation of antioxidants with viral inoculum experiment?

I would like to understand whether a specific frequency of light (in the visible spectrum) inactivates a viral species by oxidative process through ROS formation or by a conformational change of...

05 May 2024 2,109 0 View

Should I use the supernatant or lysate part of the cell?

When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...

24 March 2024 5,247 2 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

24 March 2024 466 0 View

What is the appropriate conversion factor to convert the units of measurement for antioxidants from Micromol/L to international Unit?

A For dear researchers, we have a group of variables that were obtained in different units, which are the enzyme superoxide dismutase , Glutathione and Malondialdyhyde in micromol/liter and...

18 March 2024 5,293 3 View

Can I use triphenyl tetrazolium chloride (TTC) instead of nitroblue tetrazolium (NBT) to estimate SOD activity?

Both TTC and NBT yield formazan when interacting with oxides. The superoxide dismutase assay requires NBT, which reacts with oxide to form formazan. TTC also produces Formazan when it interacts...

12 February 2024 5,006 1 View

Can we measure Superoxide dismutase activity in dried leaves?

I have dried leaves from my past experiment and need to conduct SOD activity assay. Can it be done in dried leaves?

19 January 2024 7,281 4 View

PPO (polyphenol oxidase) activity using 4-methyl catechol?

Can activity enzyme PPO (polyphenol oxidase) carried out using 4-methyl catechol in phosphate buffers pH 7,0? What wavelength can we use to see enzyme activity? Can it read at 410nm or 400nm?...

25 December 2023 1,456 1 View