Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

31 December 2018 3,925 8 View

Is using synonyms or antonyms enough in paraphrasing a text? Is doing this considered Paraphrasing?

When coming to write a research paper or a report, most EFL students tend to paraphrase through using synonyms and antonyms. Thinking that it is enough. How can we deal with such a thing?

26 December 2018 5,894 15 View

Best way to weigh hundreds of samples for a hyb ELISA?

We use a hybridization ELISA as a PK assay. We have hundreds of samples from different tissues of various treatments. Currently we're pre-weighing the tubes, then weighing the tubes with tissue...

16 August 2018 2,261 3 View

How to calculate the sample size? Can I cite the same article(s) in more than one research?

I'm planning to do a research based on questionnaire/ survey and population are clinical researchers from the whole country. The count of researchers in the country in unknown (I don't know how...

07 March 2018 2,405 4 View

In survey, not all questions answered by respondents. What should I do ?

for example, total respondents are 100. 50 answered Q6, 45 answered Q7 , 60 answered Q8. What should I do to write the results to be acceptable for the journal reviewers? The survey has...

04 February 2018 1,164 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

How do calculate decolorizing activity of bacteria by using uv-vis spectrophotometer?

27 June 2017 596 3 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

17 June 2017 5,842 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View