No, but check out Methods/Fig.5B in Nishijima et al., 2010 Neuron. They used a double-chamber well with astrocyte-endothelial cocultures in the upper chamber and neurons in lower chamber. (Having had no expertise in this in vitro prep,) I thought it was a great way to assay BBB transport of their molecule of interest (IGF-1).

Dear Prachi Anand,

                             I have little experience of converting the compound Amantadine (It is antidyskinetic medicine. It is used to treat Parkinson's disease, which is sometimes called paralysis agitans or shaking palsy. It may be given alone or with other medicines for Parkinson's disease) to cross the BBB. It is more effective than existed Amantadine salt. 

HI Amir,

Thanks for the answer, but what I am trying to know if someone knows how to make good barriers and not what can cross BBB.

 Sorry Miss. Prachi Anand the concept is any compounds which has particle size more than 100 µ can’t cross the blood brain barrier

Yes, Mr. Amir.. I know that concept.Thanks