Between therapy of acceptance and commitment and dialectical therapy behaves, which is better to build an intervention program to suicidal behavior?

I am interested in building a program from third generation therapies for factors associated with suicidal ideation and intent in university students

03 April 2019 7,322 1 View

Please, could you give a very short explanation of statistical methods used in species identific./reclassification by means of molecular markers?

This is a question on statistical methods used in DNA research Lately, many plant species are changing from species, genera, or even families due to studies in molecular makers. For example,...

07 September 2018 5,371 3 View

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10 July 2017 5,314 5 View

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06 July 2017 1,662 6 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

21 March 2016 4,523 8 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

02 March 2016 4,413 9 View

Which medium is the best for ATCC A2B5 hybridome culture?

24 January 2016 8,117 3 View

Which medium is the best for ATCC A2B5 hybridome culture?

31 December 2015 9,162 4 View

Can we freeze the trypan blue solution?

We wonder if the freezing may affect to the properties of the trypan blue. Thank you in advance!

11 December 2015 4,296 5 View

Any reason for the absence of policlonal activation of CD3+ T-cells in culture?

Hi everybody, I've been growing T cells from a flow cytometry CD3-sorting for a long time, obtained from C57BL6 mice spleens. At least three hours before seeding, the p96-well plate is coated...

08 June 2015 9,795 6 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View