Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Transfection of large plasmid ?

Hi ! Do you think can I transfect my human cells with large plasmid (15kb) ? This plasmid have lentiviral backbone, from 3rd generation lentiviral vector. I want to express transiently my...

10 December 2019 1,542 4 View

How to justify Increase in activation energy by increasing GDC/SDC?

how can we just justify the increase in activation energy by increasing the GDC/SDC content in sofc cathode..... for reference please see this paper page 6/2165.... DOI...

03 March 2019 3,132 3 View

How interface Ansys with Matlab ?

Hi! I want to execute my APDL script in a Matlab program to include it in a loop with other instructions. For first, I tried to execute my txt script which works in Ansys with this...

20 November 2017 4,923 9 View

I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents and teachers?

Hi! I'm wondering if you could help me on this? I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents ans teachers? Thank you ;)

02 November 2016 888 5 View

How to reduce aspecific recognition of denatured IgG when running WB on extracellular vesicles from plasma?

Hi everyone! I am trying to perform western blots on extracellular vesicles collected after ultracentrifugation of plasma samples. Briefly, I am diluting 250 ul of platelet-free plasma in 2 ml...

31 March 2016 9,532 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View