Transfection of large plasmid ?

Hi ! Do you think can I transfect my human cells with large plasmid (15kb) ? This plasmid have lentiviral backbone, from 3rd generation lentiviral vector. I want to express transiently my...

10 December 2019 1,542 4 View

How interface Ansys with Matlab ?

Hi! I want to execute my APDL script in a Matlab program to include it in a loop with other instructions. For first, I tried to execute my txt script which works in Ansys with this...

20 November 2017 4,923 9 View

Does anybody has an elaborated protocol for cell cycle synchronization in chinese hamster ovary cells?

04 January 2017 5,735 3 View

I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents and teachers?

Hi! I'm wondering if you could help me on this? I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents ans teachers? Thank you ;)

02 November 2016 888 5 View

How to reduce aspecific recognition of denatured IgG when running WB on extracellular vesicles from plasma?

Hi everyone! I am trying to perform western blots on extracellular vesicles collected after ultracentrifugation of plasma samples. Briefly, I am diluting 250 ul of platelet-free plasma in 2 ml...

31 March 2016 9,532 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View