Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Transfection of large plasmid ?

Hi ! Do you think can I transfect my human cells with large plasmid (15kb) ? This plasmid have lentiviral backbone, from 3rd generation lentiviral vector. I want to express transiently my...

10 December 2019 1,542 4 View

How interface Ansys with Matlab ?

Hi! I want to execute my APDL script in a Matlab program to include it in a loop with other instructions. For first, I tried to execute my txt script which works in Ansys with this...

20 November 2017 4,923 9 View

Does anybody has an elaborated protocol for cell cycle synchronization in chinese hamster ovary cells?

04 January 2017 5,735 3 View

How to reduce aspecific recognition of denatured IgG when running WB on extracellular vesicles from plasma?

Hi everyone! I am trying to perform western blots on extracellular vesicles collected after ultracentrifugation of plasma samples. Briefly, I am diluting 250 ul of platelet-free plasma in 2 ml...

31 March 2016 9,532 7 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

How can I multiply values with standard distribution and with triangular distribution?

I am calculating emissions considering the uncertainty of the data to do Monte Carlo simulation. Some variables such as yields have normal, lognormal or even uniform distributions. These must be...

03 February 2021 6,318 5 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

How can an Undergraduate collaborate with Professors globally for research projects?

I am seeking to increase my experience by working on research projects and aiming to present them as papers

01 February 2021 3,800 4 View