Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Transfection of large plasmid ?

Hi ! Do you think can I transfect my human cells with large plasmid (15kb) ? This plasmid have lentiviral backbone, from 3rd generation lentiviral vector. I want to express transiently my...

10 December 2019 1,542 4 View

How interface Ansys with Matlab ?

Hi! I want to execute my APDL script in a Matlab program to include it in a loop with other instructions. For first, I tried to execute my txt script which works in Ansys with this...

20 November 2017 4,923 9 View

Does anybody has an elaborated protocol for cell cycle synchronization in chinese hamster ovary cells?

04 January 2017 5,735 3 View

I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents and teachers?

Hi! I'm wondering if you could help me on this? I'd like to know whether there is a specific test or a tool accurate enough to assess collaboration between parents ans teachers? Thank you ;)

02 November 2016 888 5 View

How to reduce aspecific recognition of denatured IgG when running WB on extracellular vesicles from plasma?

Hi everyone! I am trying to perform western blots on extracellular vesicles collected after ultracentrifugation of plasma samples. Briefly, I am diluting 250 ul of platelet-free plasma in 2 ml...

31 March 2016 9,532 7 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How to get better phase shift in C-V curves for ferroelectric devices?

I tried to sweep my ferroelectric devices from -2V to 2V and then from 2V to -2V and The two hysteresis peaks are off by several microFarads. Should i increase bias voltage? I am doing these...

16 February 2021 8,514 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View