Hi, I will be doing patch clamp in pyramidal neurons L2/3 of V1 soon. I´ve been practising to find V1 in coronal slices with the Atlas but still I think it´s very difficult to be sure that you are in V1 and not V2.

Therefore, I wanted to ask for some advice or tricks that you use on your experiments or whether there are some differences in the morphology of V2 cells that I can use to be a bit more sure that I´m in V1.

I thought about using biotin in the pipette solution and the fixate the slices but still, it won´t be sure that I´m in V1 during the recording, only after. Furthermore, what would I take as the border between V1 and V2...?