I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible transformation of bacteria, yeast, and other microorganisms.. I wonder if it will work for plasmids or crisper cas9 !!!
This might help you
Nature Methods volume 3, pages 67–68 (2006)
doi:10.1038/nmeth0106-67
Hi Nehal, unluckily the MicroPulser is an elecroporator designed for bacterial/yeast transformation, not eukaryotic cell transfection. So it would not work with your cells, it would probably fry them.
To do eukaryotic cells you would need something like the Gene Pulser XCell (to stay with BioRad), which can do bacteria, fungi, and eukaria depending on which components you buy with the base system.
Thank you so much for your help. I found genepulser X from Bio rad and will try it.
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