I am blotting in the next couple months, but I have so many samples that it’s going to take me some time. So I wanted to know the best option for keeping my protein samples a good quality. I’ve read conflicting things regarding storing your protein samples in Laemmli’s buffer versus keeping it in it’s original lysate. Any recommendations? (I have them stored at -80°C either way)
Thanks!
you can prepare your sample with Laemmli's SDS buffer and store them at -20C
For a few weeks to a couple of months storing samples with Laemmli’s buffer at -20°C or -80°C is fine and will be ready for your experiment. The denaturation caused by the SDS will not drastically impact the quality for blotting. Aliquoting your samples to avoid freeze-thaw cycles is crucial.
For long-term storage, if you plan on storing the samples for several months or more, keeping them in their original lysate (without SDS) would be preferable to avoid potential degradation or alteration of protein conformation due to SDS denaturation.