Can anyone give me tips for using SolidWorks Flow Simulation for analysis of miscible gases?

Specifically, I have a chimney that is emitting smoke and nearby is an inlet duct that is drawing air for cooling a certain device. I need to know the percentage of smoke (contaminated air) that...

13 December 2020 5,589 2 View

What outcomes can be expected when a leader creates a positive informal environment?

I am looking for outcomes (individual or organizational) related to the creation of a positive informal work environment. Can anybody recommend me articles on the topic? Thank you in advance....

01 August 2020 7,142 3 View

MTurk and R - Calculating variable payoffs?

Hi there, I would like to run an experiment where participants can invest a % of their endowment and then lose or win 10%. This should be calculated in the background and then displayed on the...

13 January 2020 9,783 2 View

How can I compute the specific heat capacitiy in ThermoCalc?

Which commands do I use? My steps so far: - choosing the database - loading the elements - define my initial equilibriaum - set temperature as variable (step-command) and then i want to calculate...

19 December 2018 4,907 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

Quick way of measuring mitochondrial dysfunction?

Our lab is looking to measure mitochondrial dysfunction in spinal cord tissue. We think that a drug is reducing mitochondrial damage normally caused by experimental autoimmune encephalomyelitis....

30 October 2017 8,977 3 View

Suggestions for how to purify a flag tagged protein for a functional assay?

I have a protocol where they used protein G sepharose beads with another tag, but I'm not sure that protein G sepharose is compatible with the sigma flag antibody, or any other flag antibody.

25 May 2017 9,010 3 View

Has anyone administered hormones to mice through the use of creams?

I am looking to administer estriol over a long period of time (more than a year) and am considering the use of topical creams. Has anyone used this method or know of a paper or lab that has used...

22 November 2016 9,070 3 View

How do I evaluate data I got from Image Lab Normalization?

Hi, I'm the first one in my lab to use the ImageLab Normalization-tool and so far I'm not sure what to do with the data I received. I tried to standarize one blot with one protein band with a...

03 April 2016 5,179 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View