Can anyone give me tips for using SolidWorks Flow Simulation for analysis of miscible gases?

Specifically, I have a chimney that is emitting smoke and nearby is an inlet duct that is drawing air for cooling a certain device. I need to know the percentage of smoke (contaminated air) that...

13 December 2020 5,589 2 View

What outcomes can be expected when a leader creates a positive informal environment?

I am looking for outcomes (individual or organizational) related to the creation of a positive informal work environment. Can anybody recommend me articles on the topic? Thank you in advance....

01 August 2020 7,142 3 View

MTurk and R - Calculating variable payoffs?

Hi there, I would like to run an experiment where participants can invest a % of their endowment and then lose or win 10%. This should be calculated in the background and then displayed on the...

13 January 2020 9,783 2 View

How can I compute the specific heat capacitiy in ThermoCalc?

Which commands do I use? My steps so far: - choosing the database - loading the elements - define my initial equilibriaum - set temperature as variable (step-command) and then i want to calculate...

19 December 2018 4,907 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

Quick way of measuring mitochondrial dysfunction?

Our lab is looking to measure mitochondrial dysfunction in spinal cord tissue. We think that a drug is reducing mitochondrial damage normally caused by experimental autoimmune encephalomyelitis....

30 October 2017 8,977 3 View

Suggestions for how to purify a flag tagged protein for a functional assay?

I have a protocol where they used protein G sepharose beads with another tag, but I'm not sure that protein G sepharose is compatible with the sigma flag antibody, or any other flag antibody.

25 May 2017 9,010 3 View

Has anyone administered hormones to mice through the use of creams?

I am looking to administer estriol over a long period of time (more than a year) and am considering the use of topical creams. Has anyone used this method or know of a paper or lab that has used...

22 November 2016 9,070 3 View

How do I evaluate data I got from Image Lab Normalization?

Hi, I'm the first one in my lab to use the ImageLab Normalization-tool and so far I'm not sure what to do with the data I received. I tried to standarize one blot with one protein band with a...

03 April 2016 5,179 3 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View