Does anyone know of a supplier in South Africa who sells glass staining blocks / embryo dishes? Needs to be the single cavity clear glass block of 4x4cm.
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,558 0 View
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20 May 2024 7,434 0 View
I have lots of Academic Term Papers and Seminar papers which under go thorough literature reviews and are well reference but do not follow journal article guidelines. Can i publish them? Saba'atu
11 April 2024 1,729 2 View
Which software to compare two genomes
09 April 2024 4,571 2 View
Our research group is using Paclitaxel for a number of experiments and want to know if Paclitaxel can be made up and stored in media for a couple days? And if so, how long? It just feels wasteful...
09 April 2024 3,516 0 View
I have purchased a commercial grade EEG machine, for a reasonable price, in order to perform a study for my project. How familiar are you with less sophisticated EEG equipment? To what extent...
15 March 2024 9,449 5 View
We are looking for labs based in the US that assess cortisol in human hair samples. We used to send samples to Mark Laudenslager's lab at the University of Colorado for analysis. We saw another...
29 February 2024 3,225 0 View
i need to know what leadership roles used by the school heads who are assigned in two schools by using multiple role theory
12 February 2024 8,346 2 View
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14 January 2024 1,617 0 View
i am finding that drug court officers abiding by SAMSHA best practices protocol set up decades ago, have not been educated in interpreting results (they arent medical students) They look for the...
10 January 2024 440 2 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,915 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
How the damages of building and shopping centers affected the south African economy?
24 July 2024 5,401 2 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View