Does anyone know growth rate of E. coli in davis minimal 25 medium (DM25)? I have been growing the cells for 24 hours and cells are not growing well.
In plasma physics, thermodynamic property is influenced by isothermally confined electrons and adiabatically expanding electrons. How can isothermally confined electrons and adiabatically...
28 August 2023 1,740 3 View
In-vivo biochemical assays like- BCA, Nitrite, GSH, Catalase, SOD, Ca+2 assay, LDH, and many more
23 August 2023 4,724 3 View
Precambrian wrench footprints captured in composite Bouguer anomaly map of the Outrer Himalayas. India
13 August 2023 4,001 2 View
What is the reason behind farmers using chemical fertilizers?
03 August 2023 6,453 3 View
The proposed function is Y = f( Xi, Ci, Bi, Oi) Where, Dependent variable Y= Yield Independent variables Xi= Physical inputs (i.e. chemical fertilizers, organic fertilizers, etc) Ci=Climatic...
11 July 2023 589 4 View
Recently, I found some parasitic eggs in a patient's urine sample and the patient is taking immunosuppressive drugs for SLE. When a microscopic examination of the urine found the parasitic eggs...
03 April 2023 6,733 4 View
i need protocol from the specification for marine monitoring- part 4: sea water analysis. Tia
05 March 2023 7,972 1 View
When I perform cell cycle analysis using Cell Quest flow cytometer, I can not well initial gating of FSC-H and SSC-H. Because most of the cells are not see in the FSC and SSC figures. If anyone...
18 December 2022 3,360 2 View
I need to do the proteomic profiling of some of my experimental samples. Please let me know if anyone has the contact number or email ID of such service providers. Thank you!
20 October 2022 10,023 0 View
Hello, I would like to make a standard calibration curve for Acetaldehyde by GCMS in order to analyze the content of Acetaldehyde in rPET samples. We have been trying several ways. However we...
21 June 2022 6,305 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View