I need to know if Cos-7 cells naturally express Gs alpha-subunit as I've read several papers that have transfected it into these cell types
Any help would be greatly appreciated.
Jordan
For flow cytometry purposes, I am interested in treating activated, human PBMCs with a CD39 inhbitor in the presence of several concentrations of exogenous ATP and then measure changes in surface...
08 September 2018 7,892 2 View
I've peered through the literature and everything I've read is transfection of CXCR7 into Hek293 cells. So I'm assuming hek293 cells don't. Any tips would be appreciated. Thank you Jordan
04 May 2017 4,815 1 View
For growing Hek293 cells in MEM media, should the media have L-gluatmine or not?
03 April 2017 9,412 1 View
My Hek293 cells are grown in MEM media +10% FBS + 2mM glutamine. When the container the cells are in is moved, a clusty of cells would collapse upon itself. I know its not the quality of the FBS,...
03 April 2017 418 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View