I need to know if Cos-7 cells naturally express Gs alpha-subunit as I've read several papers that have transfected it into these cell types
Any help would be greatly appreciated.
Jordan
For flow cytometry purposes, I am interested in treating activated, human PBMCs with a CD39 inhbitor in the presence of several concentrations of exogenous ATP and then measure changes in surface...
08 September 2018 7,834 2 View
I've peered through the literature and everything I've read is transfection of CXCR7 into Hek293 cells. So I'm assuming hek293 cells don't. Any tips would be appreciated. Thank you Jordan
04 May 2017 4,755 1 View
For growing Hek293 cells in MEM media, should the media have L-gluatmine or not?
03 April 2017 9,295 1 View
My Hek293 cells are grown in MEM media +10% FBS + 2mM glutamine. When the container the cells are in is moved, a clusty of cells would collapse upon itself. I know its not the quality of the FBS,...
03 April 2017 350 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View