Jordan Boeck

5 Questions 1 Answers 0 Followers

Questions related from Jordan Boeck

What surface receptors in T cells are affected by exogenous ATP?

For flow cytometry purposes, I am interested in treating activated, human PBMCs with a CD39 inhbitor in the presence of several concentrations of exogenous ATP and then measure changes in surface...

09 September 2018 7,873 2 View

Do Hek293 cells naturally express CXCR7?

I've peered through the literature and everything I've read is transfection of CXCR7 into Hek293 cells. So I'm assuming hek293 cells don't. Any tips would be appreciated. Thank you Jordan

05 May 2017 4,797 1 View

I'm growing Hek293 cells, and they are loosely attached to the container they are in. What could be the problem?

My Hek293 cells are grown in MEM media +10% FBS + 2mM glutamine. When the container the cells are in is moved, a clusty of cells would collapse upon itself. I know its not the quality of the FBS,...

04 April 2017 405 3 View

For growing Hek293 cells in MEM media, should the media have L-gluatmine or not?

04 April 2017 9,382 1 View

Do you know if Cos-7 (monkey kidney fibroblasts) cells express the Gs alpha-subunit?

I need to know if Cos-7 cells naturally express Gs alpha-subunit as I've read several papers that have transfected it into these cell types Any help would be greatly appreciated. Jordan

05 May 2016 2,865 0 View