For growing Hek293 cells in MEM media, should the media have L-gluatmine or not?
Hi, If your MEM medium already inlcudes L-Glutamine then not, else you need to add it.
For flow cytometry purposes, I am interested in treating activated, human PBMCs with a CD39 inhbitor in the presence of several concentrations of exogenous ATP and then measure changes in surface...
08 September 2018 7,892 2 View
I've peered through the literature and everything I've read is transfection of CXCR7 into Hek293 cells. So I'm assuming hek293 cells don't. Any tips would be appreciated. Thank you Jordan
04 May 2017 4,815 1 View
My Hek293 cells are grown in MEM media +10% FBS + 2mM glutamine. When the container the cells are in is moved, a clusty of cells would collapse upon itself. I know its not the quality of the FBS,...
03 April 2017 418 3 View
I need to know if Cos-7 cells naturally express Gs alpha-subunit as I've read several papers that have transfected it into these cell types Any help would be greatly appreciated. Jordan
04 May 2016 2,880 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View