Hi all,
I want to ask about my cell culture. My cell is PK-15 cell line. When they are already reach the confluency, I noticed there are several rounded cells grouped and formed brown spots. Do you think this is normal or anything should I concerned (contaminants, etc)?
Is there any branchy/grape-like particles floating in media? Do you observing typical signs of contamination (turbidity of media, acidic pH)?
If no - your cells are 99% fine.
Hi Dmitry,
No, the media was just fine. However, since the first time we acquired this cell line, it always has a quite amount of debris. Not branchy/grape-like, but rather single cell particle-like.
Hello! It was 2 weeks since your question - are your cells still fine? Do they exhibit normal PDT and morphology?
Hi Dmitry,
Yes, actually this condition did not affect anything related to cell growth. The doubling time is just same as before. I hope that brown spot is just because overgrown cells, as you said before.
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