Khrisna Putera Buddhiman

5 Questions 13 Answers 0 Followers

Questions related from Khrisna Putera Buddhiman

Do you have opinion of these brown spots on my PK-15 cells?

Hi all, I want to ask about my cell culture. My cell is PK-15 cell line. When they are already reach the confluency, I noticed there are several rounded cells grouped and formed brown spots. Do...

12 December 2017 1,987 6 View

DEAE Dextran for virus infection to cell?

Hi all, As long as I know, DEAE Dextran can mediate the delivery of nucleic acid into mammalian cells during transfection process. Can DEAE Dextran also mediate the delivery/internalization of the...

05 May 2017 315 4 View

Roller bottle cell culture: Use CO2 or not?

Hello all, We want to scale up cell culture facilities in our lab from flask to roller bottle. Usually we use CO2 incubator for cell culturing activity in cell culture flask. My question is...how...

11 November 2016 743 7 View

What is the difference between Vero and MARC 145 cell line?

Hello all! What is the difference between Vero and MARC 145 cell line? Several literature said they are both taken from monkey's kidney. I will use this for virus inoculation application...Thank...

09 September 2016 2,515 4 View

What's the reason behind SKOV3 cells shape change?

I'm trying to culture SKOV3 cells with RPMI 1640 medium+10% FBS+insulin+PenStrep. Something unusual happened after my first splitting with 1:2 ratio using Trypsin & EDTA. The shape of the...

01 January 2015 1,709 5 View