Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

Standarized vs. non standarized beta?

in the analysing the structural model in SEM using smart PLS , what is the standarized , and the non-standaraized beta in the outcome tables? cause in the path coefficient headline , there is...

26 October 2020 3,930 3 View

What to do if path coefficient is significant and f2 is no effect ? p and f2 contradicts?

in my phd thesis analysis through PLS for 574 sample size , is it fine to have significant path coefficient between A and B variables, but f2 less than .02 in the same time? specifically .189, how...

15 April 2020 2,728 3 View

Carbon weight percent in austenite?

Hello, I'm a first year student, we have a steel alloy that consists of 0.6 wt.% carbon, but when we measured the carbon content in austenite using XRD, we got 1.30 wt.% carbon. How can...

14 April 2019 9,870 4 View

How to deal with absent of standardization in methodology to carry-out new study?

Hi, I will be using image processing techniques to predict treatment outcomes to some kind of tumors. I'm about to design my study, So, I went through many papers and literature that has...

25 March 2019 2,348 2 View

Transfection of primary human hepatocytes with Lipofectamine 3000 - any success stories?

Was there something you absolutely needed to change in the general protocol to succeed? I would really appreciate some insight from people with first hand experience.

11 December 2017 5,757 9 View

Do you have first hand experience with a DLL4 (or any other Notch-ligand) antibody for IF/ICC ?

We would like to stain a monolayer of adherent cells. I would appreciate some advice from people who successfully used a particular brand/product.

04 May 2017 6,953 0 View

DAB Enhancer from Abcam (ab675) - has it helped your IHC?

We are staining mouse liver sections, the detection system is HRP-DAB. The pictures are looking good but you know how it is, the signal could always be a bit stronger. I'd like to hear from people...

31 December 2015 3,889 6 View

Can someone provide me with references about stormwater biofiltration structures at construction sites?

Do you have examples about publications discussing stormwater biofiltration during construction works? I think I have heard that if biofiltration or any other vegetated stormwater management...

19 February 2015 5,132 3 View

Has anyone used Genewiz for DNA synthesis?

02 June 2014 9,259 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View