We would like to stain a monolayer of adherent cells. I would appreciate some advice from people who successfully used a particular brand/product.
Hello, I recently updated my MacBook to Sonoma and after installing the newest Java version which was necessary to get Mplus to run, everything looked fine. But now when I want to run an input...
09 April 2024 7,783 1 View
what is the procedure of synthesis of nanomaterial -Bottom-up -Chemical Etching? Advantage and disadvantagesAdvantages of this technique?
04 April 2024 2,522 2 View
Hi everyone, I was recently doing a simulation and actual measurement of a rectifier circuit, using the series diode SMS7630-079LF (Breakdown Voltage=2V). The output DC voltage obtained by my...
21 March 2024 1,166 2 View
I am doing BMDMs and I can not detach macrophages , they are so sticky and trypsin is not enough to detach them. would scrapper works or will it destroy the cells ?
07 March 2024 2,502 3 View
We are looking for collaborations for doctoral research.
29 January 2024 5,216 2 View
My object is to create a shapefile to distinguish between geological features and artificial features.
22 January 2024 9,435 6 View
SNAP is mainly for sentinel image preprocessing. The tasks like reprojection, and sampling—would they bring about the same result if done in ArcGIS?
22 January 2024 3,376 3 View
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29 December 2023 4,732 0 View
What is recent research on fentanyl and its derivatives determination by differential Raman spectroscopy?
15 December 2023 9,912 0 View
what is the best software for paraphrasing English texts?
11 December 2023 836 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View