Hello everyone,
Does anyone have a working protocol for transfection on MAC-T using lipofectamine? Thanks in advance for any help, Aly
I am working with virus and I need to determine my virus titer. On my assay, I use MTT. I got a grafic from a coworker and I am struggled to understand a grafic showing the results. I really...
06 July 2017 3,545 0 View
I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it...
01 February 2015 1,608 4 View
I am going to collect blood cells, but I am having some problems with my -80C freezer. So, I am planning to add RNA later (Ambion) on my samples (blood cells) and keep them at -20C until RNA...
05 June 2014 8,243 3 View
I designed a primer for my target using a sequence published by NCBI (GenBank: FJ900270.1). However, BLASTing primers sequences on ENSEMBL BLAST tool, the browser showed that forward sequences is...
31 December 2013 4,825 14 View
I extracted total RNA from my samples and I ran an agarose gel 2%. You will see two bands on the top of the gel, and one strong band on the bottom, which is my loading dye (bromophenol blue), and...
05 June 2013 4,469 3 View
A friend of mine is looking for carborundum. Can I buy this from some company?
02 March 2013 7,122 0 View
I am doing RNA extraction using Trizol Reagent from Invitrogen. I got rations A260/280 1.60 and A260/230 1.19. Is it possible to clean up my samples and increase my rations? Is it useful to...
01 February 2013 8,579 15 View
I have been getting contamination in my PCR reactions. Because I could not eliminate the contamination internally, I bought new a pipet, new filtered tips, new SYBR, new water and new primers....
31 December 2012 9,479 43 View
Is there some restriction and/or disadvantage?
10 November 2012 9,450 2 View
I am trying to isolate RNA from sperm using Trizol, but I can not get good results. I have some protocols to give a try and they suggest keep the sample + Trizol on ice. However, I had seen some...
08 September 2012 3,715 17 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View