Viral titer calculation

06 July 2017 3,470 0 View

Does anyone know if I can reverse denaturation by urea (7M)?

I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it...

01 February 2015 1,513 4 View

Can I use RNA later to preserve blood cells at -20C? If yes, how much should I use?

I am going to collect blood cells, but I am having some problems with my -80C freezer. So, I am planning to add RNA later (Ambion) on my samples (blood cells) and keep them at -20C until RNA...

05 June 2014 8,169 3 View

Can my primer amplify if the forward is located in an intron region?

I designed a primer for my target using a sequence published by NCBI (GenBank: FJ900270.1). However, BLASTing primers sequences on ENSEMBL BLAST tool, the browser showed that forward sequences is...

31 December 2013 4,726 14 View

What does the bright/shiny band on the bottom of the RNA gel indicate?

I extracted total RNA from my samples and I ran an agarose gel 2%. You will see two bands on the top of the gel, and one strong band on the bottom, which is my loading dye (bromophenol blue), and...

05 June 2013 4,355 3 View

Does anybody have experience with carborundum?

A friend of mine is looking for carborundum. Can I buy this from some company?

02 March 2013 7,051 0 View

RNA samples with low A260/280 and A260/230 ratios

I am doing RNA extraction using Trizol Reagent from Invitrogen. I got rations A260/280 1.60 and A260/230 1.19. Is it possible to clean up my samples and increase my rations? Is it useful to...

01 February 2013 8,319 15 View

Why do I get amplification curves for my qPCR run without a template?

I have been getting contamination in my PCR reactions. Because I could not eliminate the contamination internally, I bought new a pipet, new filtered tips, new SYBR, new water and new primers....

31 December 2012 9,387 43 View

May I use DEPC water for qPCR?

Is there some restriction and/or disadvantage?

10 November 2012 9,335 2 View

Sperm RNA isolation using Trizol

I am trying to isolate RNA from sperm using Trizol, but I can not get good results. I have some protocols to give a try and they suggest keep the sample + Trizol on ice. However, I had seen some...

08 September 2012 3,650 17 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View