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Questions related from Aletheia Lima Souza
I am working with virus and I need to determine my virus titer. On my assay, I use MTT. I got a grafic from a coworker and I am struggled to understand a grafic showing the results. I really...
07 July 2017 3,506 0 View
Hello everyone, Does anyone have a working protocol for transfection on MAC-T using lipofectamine? Thanks in advance for any help, Aly
10 October 2015 1,684 0 View
I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it...
02 February 2015 1,557 4 View
I am going to collect blood cells, but I am having some problems with my -80C freezer. So, I am planning to add RNA later (Ambion) on my samples (blood cells) and keep them at -20C until RNA...
06 June 2014 8,211 3 View
I designed a primer for my target using a sequence published by NCBI (GenBank: FJ900270.1). However, BLASTing primers sequences on ENSEMBL BLAST tool, the browser showed that forward sequences is...
01 January 2014 4,787 14 View
I extracted total RNA from my samples and I ran an agarose gel 2%. You will see two bands on the top of the gel, and one strong band on the bottom, which is my loading dye (bromophenol blue), and...
06 June 2013 4,432 3 View
A friend of mine is looking for carborundum. Can I buy this from some company?
03 March 2013 7,095 0 View
I am doing RNA extraction using Trizol Reagent from Invitrogen. I got rations A260/280 1.60 and A260/230 1.19. Is it possible to clean up my samples and increase my rations? Is it useful to...
02 February 2013 8,509 15 View
I have been getting contamination in my PCR reactions. Because I could not eliminate the contamination internally, I bought new a pipet, new filtered tips, new SYBR, new water and new primers....
01 January 2013 9,441 43 View
Is there some restriction and/or disadvantage?
11 November 2012 9,424 2 View
I am trying to isolate RNA from sperm using Trizol, but I can not get good results. I have some protocols to give a try and they suggest keep the sample + Trizol on ice. However, I had seen some...
09 September 2012 3,690 17 View
I ran qPCR using two primers for my target genes in different tissues. I got sharp bands for one primers, but not the other, I had smeared bands. What happened with the cDNA? Degraded RNA could be...
08 August 2012 2,071 8 View
I am looking for a PCR machine for my lab. Which specifications are most important? And what does it mean when the thermal is Peltier-based designed?
07 July 2012 747 33 View
