A friend of mine is looking for carborundum. Can I buy this from some company?
I am working with virus and I need to determine my virus titer. On my assay, I use MTT. I got a grafic from a coworker and I am struggled to understand a grafic showing the results. I really...
06 July 2017 3,545 0 View
Hello everyone, Does anyone have a working protocol for transfection on MAC-T using lipofectamine? Thanks in advance for any help, Aly
09 October 2015 1,720 0 View
I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it...
01 February 2015 1,608 4 View
I am going to collect blood cells, but I am having some problems with my -80C freezer. So, I am planning to add RNA later (Ambion) on my samples (blood cells) and keep them at -20C until RNA...
05 June 2014 8,243 3 View
I designed a primer for my target using a sequence published by NCBI (GenBank: FJ900270.1). However, BLASTing primers sequences on ENSEMBL BLAST tool, the browser showed that forward sequences is...
31 December 2013 4,825 14 View
I extracted total RNA from my samples and I ran an agarose gel 2%. You will see two bands on the top of the gel, and one strong band on the bottom, which is my loading dye (bromophenol blue), and...
05 June 2013 4,469 3 View
I am doing RNA extraction using Trizol Reagent from Invitrogen. I got rations A260/280 1.60 and A260/230 1.19. Is it possible to clean up my samples and increase my rations? Is it useful to...
01 February 2013 8,579 15 View
I have been getting contamination in my PCR reactions. Because I could not eliminate the contamination internally, I bought new a pipet, new filtered tips, new SYBR, new water and new primers....
31 December 2012 9,479 43 View
Is there some restriction and/or disadvantage?
10 November 2012 9,450 2 View
I am trying to isolate RNA from sperm using Trizol, but I can not get good results. I have some protocols to give a try and they suggest keep the sample + Trizol on ice. However, I had seen some...
08 September 2012 3,715 17 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View