I'd like to make some Wnt/R-Sponding media to lower the costs of organoid maintenance
Many thanks
http://www.nature.com/nprot/journal/v10/n10/full/nprot.2015.088.html
There is a protocol for conditioned media in here. Good luck!
Thanks
This paper is a very detailed Nat Protocol on preparing organoid culture media
http://www.nature.com/nprot/journal/v8/n12/abs/nprot.2013.153.html
I have used it and it works well. Best luck!
I addition to my previous answer, check this paper as well. Hope it helps.
https://www.ncbi.nlm.nih.gov/pubmed/30548444
Generation of Pancreatic Ductal Organoids and Whole-Mount Immunostaining of Intact Organoids.
I am learning to culture organoids. Many thanks for the updating information.
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