If E-cad and Vimentin increased simultaneously, how to interpret?

I am doing research focusing on EMT, but I found that E-cad and Vimentin increased simultaneously frequently after treatment. How to interpret this phenomenon? Do guys you have some literature to...

06 July 2018 6,115 0 View

When to Add the autophagy treament in cell culture models?

I am doing Exps on autophagy mechanisms. A question is that: when to add autophagy treatment (CQ, 3-MA, Rapa) into cultre models? Immediately when seeding? Or after the cells adhesion into a...

01 February 2018 2,040 4 View

How to set transwell control after transfection?

Hi I am doing the research linking autophagy and EMT, the factor is HBV infection. Now I am doing a experiment like this: first transfected Huh751 cells with HBX, 48 hrs later, seeding in...

01 February 2018 5,043 2 View

Do you know some Free graph app in mac system?

I am planing to do some graph about PCR results. Do you know some free apps, for doing a good graph in mac system?

31 December 2017 7,123 0 View

How to maintain cell status in cell culture when passage too many times?

I am doing cell transfection with plasmid DNA. I found that the transfection efficiency was related to the cell status definitely. For example, I used Huh7 cells these days. When cells were in...

10 November 2017 4,590 3 View

Plasmid transfection in Huh7 cells with FuGENE reagent, do you have a good protocol?

Now I am developing transfection protocol on transfection efficiency of Huh7 cells. Reagent:FuGene HD. Plasmid:GFP DNA. I found that Huh7 was easy to be transfected. Unfortunately my huh7 cells...

10 November 2017 2,687 8 View

Transfection rate with GFP: how to improve the efficiency?

I am a new guy in scientific research. These days I am trying to start pre-experiment of transfection. Protocol summary: 24-wells, HepG2 & Huh-7 cells, 10000 cells/well, FuGENE or LTX reagent,...

09 October 2017 9,317 4 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View