Dong Chen

8 Questions 5 Answers 0 Followers

Questions related from Dong Chen

If E-cad and Vimentin increased simultaneously, how to interpret?

I am doing research focusing on EMT, but I found that E-cad and Vimentin increased simultaneously frequently after treatment. How to interpret this phenomenon? Do guys you have some literature to...

07 July 2018 6,197 0 View

How to set transwell control after transfection?

Hi I am doing the research linking autophagy and EMT, the factor is HBV infection. Now I am doing a experiment like this: first transfected Huh751 cells with HBX, 48 hrs later, seeding in...

02 February 2018 5,114 2 View

When to Add the autophagy treament in cell culture models?

I am doing Exps on autophagy mechanisms. A question is that: when to add autophagy treatment (CQ, 3-MA, Rapa) into cultre models? Immediately when seeding? Or after the cells adhesion into a...

02 February 2018 2,076 4 View

Do you know some Free graph app in mac system?

I am planing to do some graph about PCR results. Do you know some free apps, for doing a good graph in mac system?

01 January 2018 7,171 0 View

Do you have a good cell model for the relationship investigation between HBV and hepatocellular carcinoma?

Hi I am doing some research work on the relationship between HBV and hepatocellular carcinoma. What I am doing includes: G2/AD38 system, G2 (or H751)/HBX transfection system, H751-ntcp or NTCP...

01 January 2018 4,653 5 View

Plasmid transfection in Huh7 cells with FuGENE reagent, do you have a good protocol?

Now I am developing transfection protocol on transfection efficiency of Huh7 cells. Reagent:FuGene HD. Plasmid:GFP DNA. I found that Huh7 was easy to be transfected. Unfortunately my huh7 cells...

11 November 2017 2,767 8 View

How to maintain cell status in cell culture when passage too many times?

I am doing cell transfection with plasmid DNA. I found that the transfection efficiency was related to the cell status definitely. For example, I used Huh7 cells these days. When cells were in...

11 November 2017 4,648 3 View

Transfection rate with GFP: how to improve the efficiency?

I am a new guy in scientific research. These days I am trying to start pre-experiment of transfection. Protocol summary: 24-wells, HepG2 & Huh-7 cells, 10000 cells/well, FuGENE or LTX reagent,...

10 October 2017 9,435 4 View