I am doing research focusing on EMT, but I found that E-cad and Vimentin increased simultaneously frequently after treatment. How to interpret this phenomenon? Do guys you have some literature to explain or explore on this issue?
thanks
I am doing Exps on autophagy mechanisms. A question is that: when to add autophagy treatment (CQ, 3-MA, Rapa) into cultre models? Immediately when seeding? Or after the cells adhesion into a...
01 February 2018 2,091 4 View
Hi I am doing the research linking autophagy and EMT, the factor is HBV infection. Now I am doing a experiment like this: first transfected Huh751 cells with HBX, 48 hrs later, seeding in...
01 February 2018 5,140 2 View
Hi I am doing some research work on the relationship between HBV and hepatocellular carcinoma. What I am doing includes: G2/AD38 system, G2 (or H751)/HBX transfection system, H751-ntcp or NTCP...
31 December 2017 4,669 5 View
I am planing to do some graph about PCR results. Do you know some free apps, for doing a good graph in mac system?
31 December 2017 7,187 0 View
I am doing cell transfection with plasmid DNA. I found that the transfection efficiency was related to the cell status definitely. For example, I used Huh7 cells these days. When cells were in...
10 November 2017 4,670 3 View
Now I am developing transfection protocol on transfection efficiency of Huh7 cells. Reagent:FuGene HD. Plasmid:GFP DNA. I found that Huh7 was easy to be transfected. Unfortunately my huh7 cells...
10 November 2017 2,786 8 View
I am a new guy in scientific research. These days I am trying to start pre-experiment of transfection. Protocol summary: 24-wells, HepG2 & Huh-7 cells, 10000 cells/well, FuGENE or LTX reagent,...
09 October 2017 9,458 4 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
How can I interpret the data gathered without solving?
03 August 2024 9,054 3 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...
23 July 2024 2,269 1 View
This is a analysis on IHC scoring of WNVs and USUV performed with culex pipiens
22 July 2024 1,880 0 View
Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...
20 July 2024 5,676 3 View
I am struggling to attain multiple research articles, so far, I have looked at the Swedish longitudinal study, they have good depth and reliability. I was thinking of looking into twin studies to...
15 July 2024 3,737 1 View
Hi guys, in the context of my master thesis i analyze the statistical relationship between income and subjective well-being (Panel: SOEP, n: 300.000 observations over 10 years). After creating a...
13 July 2024 7,539 6 View