I am planing to do some graph about PCR results.
Do you know some free apps, for doing a good graph in mac system?
I am doing research focusing on EMT, but I found that E-cad and Vimentin increased simultaneously frequently after treatment. How to interpret this phenomenon? Do guys you have some literature to...
06 July 2018 6,115 0 View
I am doing Exps on autophagy mechanisms. A question is that: when to add autophagy treatment (CQ, 3-MA, Rapa) into cultre models? Immediately when seeding? Or after the cells adhesion into a...
01 February 2018 2,040 4 View
Hi I am doing the research linking autophagy and EMT, the factor is HBV infection. Now I am doing a experiment like this: first transfected Huh751 cells with HBX, 48 hrs later, seeding in...
01 February 2018 5,043 2 View
Hi I am doing some research work on the relationship between HBV and hepatocellular carcinoma. What I am doing includes: G2/AD38 system, G2 (or H751)/HBX transfection system, H751-ntcp or NTCP...
31 December 2017 4,612 5 View
I am doing cell transfection with plasmid DNA. I found that the transfection efficiency was related to the cell status definitely. For example, I used Huh7 cells these days. When cells were in...
10 November 2017 4,590 3 View
Now I am developing transfection protocol on transfection efficiency of Huh7 cells. Reagent:FuGene HD. Plasmid:GFP DNA. I found that Huh7 was easy to be transfected. Unfortunately my huh7 cells...
10 November 2017 2,687 8 View
I am a new guy in scientific research. These days I am trying to start pre-experiment of transfection. Protocol summary: 24-wells, HepG2 & Huh-7 cells, 10000 cells/well, FuGENE or LTX reagent,...
09 October 2017 9,317 4 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?
01 March 2021 9,383 1 View