I am planing to do some graph about PCR results.
Do you know some free apps, for doing a good graph in mac system?
I am doing research focusing on EMT, but I found that E-cad and Vimentin increased simultaneously frequently after treatment. How to interpret this phenomenon? Do guys you have some literature to...
06 July 2018 6,216 0 View
I am doing Exps on autophagy mechanisms. A question is that: when to add autophagy treatment (CQ, 3-MA, Rapa) into cultre models? Immediately when seeding? Or after the cells adhesion into a...
01 February 2018 2,091 4 View
Hi I am doing the research linking autophagy and EMT, the factor is HBV infection. Now I am doing a experiment like this: first transfected Huh751 cells with HBX, 48 hrs later, seeding in...
01 February 2018 5,140 2 View
Hi I am doing some research work on the relationship between HBV and hepatocellular carcinoma. What I am doing includes: G2/AD38 system, G2 (or H751)/HBX transfection system, H751-ntcp or NTCP...
31 December 2017 4,669 5 View
I am doing cell transfection with plasmid DNA. I found that the transfection efficiency was related to the cell status definitely. For example, I used Huh7 cells these days. When cells were in...
10 November 2017 4,670 3 View
Now I am developing transfection protocol on transfection efficiency of Huh7 cells. Reagent:FuGene HD. Plasmid:GFP DNA. I found that Huh7 was easy to be transfected. Unfortunately my huh7 cells...
10 November 2017 2,786 8 View
I am a new guy in scientific research. These days I am trying to start pre-experiment of transfection. Protocol summary: 24-wells, HepG2 & Huh-7 cells, 10000 cells/well, FuGENE or LTX reagent,...
09 October 2017 9,458 4 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View