After receiving MCF-7 cells from NCCS (India), I supplemented the flask with growth media (DMEM in 1% penicillin & streptomycin with 10% FBS). I then passaged MCF-7 cells. After 3 days, the cells look as per images attached. Both the images are taken from the same 25cc flask.
Do the cells show the expected morphology ?
Thank you in advance for your replies !
They don't have the morphology of MCF7 cells from the ATCC.
See photos: https://www.atcc.org/products/htb-22
Perhaps that is due to your culture medium. The recommended medium is EMEM + Insulin + FCS
https://www.atcc.org/atcc/productsheetpdf/generatehtmlpdf/htb-22
They look a like my mcf 7 cells after treatment...
My suggestion please
1. check the number of passage
2. wait until its confluent 🙏
This looks like a mixture of MCF-7 cells that have and have not attached to the flask. Non-attached cells will be rounded up and attached cells will be flatter. As they become more confluent, attached MCF-7 cells will take on a ”fried egg” appearance. I suggest that you let the cells grow to increased confluence to see how they behave. There are lots of pics of these cells in the old literature. Keep in mind that these cells express estrogen receptor and are estrogen sensitive. Unstripped serum and phenol red containing DMEM contain estradiol and/or other estrogenic chemicals that stimulate cell proliferation to varying levels. You can test the cells for these responses, which are inhibited by anti-estrogens (tamoxifen, Raloxifene). Hope this helps.
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