Namritha Mp

4 Questions 9 Answers 0 Followers

Questions related from Namritha Mp

Do the MCF-7 cells show this morphology when grown in a flask ?

After receiving MCF-7 cells from NCCS (India), I supplemented the flask with growth media (DMEM in 1% penicillin & streptomycin with 10% FBS). I then passaged MCF-7 cells. After 3 days, the cells...

20 August 2024 6,221 6 View

Can I use tissue culture treated flask for MCF7 cell line ?

I will be assisting my colleague to grow MCF7 cells. 75cc flasks need to ordered first. Will this flask work for MCF7 cells...

09 July 2024 9,647 4 View

How can I improve A260/A230 ratio of DNA extracted from cattle semen samples ?

I am performing phenol-chloroform-isoamyl alcohol DNA extraction method for cattle semen samples. I wash the pellet with 70% ethanol, air-dry/dry in incubator at 37deg and dissolve the pellet in...

02 July 2024 3,232 9 View

What are the points to be considered for growing MCF7 cells and performing the MTT assay ??

I will be assisting my colleague with MCF7 subculture and later performing MTT assay. We will be using DMEM media (with phenol red) along with 10% FBS and 1% penicillin/streptomycin. We will...

13 June 2024 1,133 3 View