Do SH-SY5Y cells fire spontaneous action potentials?

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ORCA calculations. Applying pressure during geometry optimization, is it possible?

Dear All, For my research I'd like to use DFT computation in the ORCA program. I'd like to calculate the changing of bandgap (value calculated from HOMO and LUMO energy) of a molecule depending...

22 October 2022 4,299 3 View

What range and sensitivity should I choose for Human Elisa Kit GPX1 to get the optimal result after double wingate test 24 / 48h after ?

Kit which company do you recommend for ELISA after physical exercise in people ?

07 June 2020 8,752 0 View

I am looking for protocol for pH measurments in liquid using orange acridine (pH range 5-7). If someone has this protocol tahat would be grat! thanks

I treat arabidopsis seedlings in liquid culture with flagellin and want to track pH changes over 40 min by taking aliquots of 100 ul and measuring pH. Any other idea to do so is appriciated. Thanks

17 May 2017 9,506 1 View

Why the pH of Citrate buffer increases when diluted with water?

I have made up a 1M Citrate buffer at pH 4.5. I have then diluted this solution with deionised water to give concentrations of 50 mM, 75 mM and 100 mM and measured their pH. I've got the following...

22 July 2015 3,194 14 View

Which statistics should be used to test the interrelationships between coping styles (avoiding, emotional and problem focused) and burnout?

Could anyone help with a bit of statistics: There are three coping styles in the research (emotion focused, problem focused and avoidance oriented) and three dimensions of burnout (emotional...

22 September 2014 6,310 3 View

Why does oxygen content in biochars decrease their toxicity?

We've observed a decrease in phytotoxicity (3 day solid phase test - Phytotoxicity FTM) of biochars with higher oxygen content comparing to biochar with lower oxygen content. Does anyone have an...

22 May 2014 6,079 3 View

Which years were crucial for CEECs in terms of trade liberalisation with the EU before 2004 eastward enlargement? I'd be grateful for some literature

I have some intuition that the pre-accession period was very important in shaping the CEECs trade with the EU. I'd be grateful for some detailed information/sources how the gradual trade...

02 September 2013 3,548 2 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to use ERP System in Global University ?

27 July 2024 8,229 0 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

Co-culture of SH-SY5Y cells and astrocytes?

Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...

01 July 2024 243 2 View

How to do single-trial ERP analysis?

Single-trial ERP analysis provides a way to extract the amplitude of each trial rather than one certain condition...

01 July 2024 6,553 2 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

What are the best fsc/ssc settings for flow cytometry of Chamydomonas?

We are having trouble getting the settings right on a FACS Canto to see a nice population of cells.

20 June 2024 4,132 0 View

Action research design is Quantitative Studies or Qualitative Studies?

14 June 2024 1,684 1 View

Is it possible to do FACS on snap frozen cells ?

Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.

11 June 2024 8,915 0 View