What range and sensitivity should I choose for Human Elisa Kit GPX1 to get the optimal result after double wingate test 24 / 48h after ?

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Kit which company do you recommend for ELISA after physical exercise in people ?

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Do SH-SY5Y cells fire spontaneous action potentials?

I'm currently culturing differentiated and undifferentiated SH-SY5Ys for calcium imaging with Fluo-4AM. Do SH-SY5Y cells spontaneously fire action potentials, or do they need to be stimulated?

30 November 2022 3,653 2 View

ORCA calculations. Applying pressure during geometry optimization, is it possible?

Dear All, For my research I'd like to use DFT computation in the ORCA program. I'd like to calculate the changing of bandgap (value calculated from HOMO and LUMO energy) of a molecule depending...

22 October 2022 4,299 3 View

I am looking for protocol for pH measurments in liquid using orange acridine (pH range 5-7). If someone has this protocol tahat would be grat! thanks

I treat arabidopsis seedlings in liquid culture with flagellin and want to track pH changes over 40 min by taking aliquots of 100 ul and measuring pH. Any other idea to do so is appriciated. Thanks

17 May 2017 9,506 1 View

Why the pH of Citrate buffer increases when diluted with water?

I have made up a 1M Citrate buffer at pH 4.5. I have then diluted this solution with deionised water to give concentrations of 50 mM, 75 mM and 100 mM and measured their pH. I've got the following...

22 July 2015 3,194 14 View

Which statistics should be used to test the interrelationships between coping styles (avoiding, emotional and problem focused) and burnout?

Could anyone help with a bit of statistics: There are three coping styles in the research (emotion focused, problem focused and avoidance oriented) and three dimensions of burnout (emotional...

22 September 2014 6,310 3 View

Why does oxygen content in biochars decrease their toxicity?

We've observed a decrease in phytotoxicity (3 day solid phase test - Phytotoxicity FTM) of biochars with higher oxygen content comparing to biochar with lower oxygen content. Does anyone have an...

22 May 2014 6,079 3 View

Which years were crucial for CEECs in terms of trade liberalisation with the EU before 2004 eastward enlargement? I'd be grateful for some literature

I have some intuition that the pre-accession period was very important in shaping the CEECs trade with the EU. I'd be grateful for some detailed information/sources how the gradual trade...

02 September 2013 3,548 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

What are the principles of physical chemistry?

what are the main points

10 July 2024 9,236 5 View

Is there any materials that can be as flexible and stretchable as PDMS, while also having a small linear thermal expansion coefficient?

For the PDMS material, it's linear thermal expansion coefficient is about 3.2*10-4/℃. The polyimide has a smaller linear thermal expansion coefficient like 2*10-5/℃, but it cannot be stretch. It...

04 July 2024 4,525 1 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View