DNA Gel Electrophoresis Image – Smearing and Band Clarity Concerns: What could be the reason for the smearing,I made use of SYBR green and 1% agarose?

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Which are the scales published regarding the decision-making for retirement. In another words which are the options workers have facing retirement?

We have many scales that describe the predictors (or independent variables) older workers have to push or pull them on the transition from work to retirement. Considering that retirement decision...

16 September 2023 5,224 1 View

Do you know any converter, script or program that convert segd to segy format available?

I would like to convert segd format to segy.

07 May 2023 4,339 2 View

Can I ask some examples of narratology research if any or some related examples?

Confusions on some of my research that relates to a qualitative one.

11 June 2021 7,048 8 View

Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligo nucleotide in solid phase?

Hi, anybody help me? Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligonucleotide in solid phase? What's the consequences?

28 July 2020 8,312 3 View

How to use InSAR data for determining the ground's surface motion?

Does anyone have a tutorial or paper (for beginners) on using InSAR data for calculating the motion of sudden or gradual sinking of the ground's surface (subsidence) with free software? I really...

15 July 2019 1,695 2 View

Has anyone worked with 3 PGIS Model? Or the 3PG Spatial for ArcGis platform? Could you share the experience and what was applied?

Especially with application for forests. Thanks!

13 February 2018 8,740 0 View

Do anyone know how to create circular amostrage graphic?

I want to create the same graph like the image from this paper (Sci Transl Med. 2016 Dec 14;8(369):369ra176) Thank you

05 February 2018 3,915 2 View

What is the correct method to calculate the spread of a ensamble of a few elements?

I have an ensemble of 3 reanalysis. What is the correct method to calculate the spread of the ensemble? I doubt between to apply inter-reanalysis standard deviation or the range (max - min) of the...

23 May 2017 9,605 2 View

Has any one a Scale that measures the reasons for migration in retirees who are searchinf for increasing their f well-being?

I am starting a research that investigates the correlation of the reasons for migration befote to move in retirement and the well-being after the change? Could anyone help me with instruments and...

31 August 2016 2,627 9 View

Why the transfection efficiency measured by flow cytometry is not coupled to the real-time data ?

I performed a transient transfection of premiR , I have analyzed the efficiency of transfection by flow cytometry after 24 , 48 and 72 hours after transfection through the analysis of cells...

06 March 2016 1,301 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View