Any advice on selecting human or animal studies to conduct a systematic review?

Human studies are more relevant to the project, however there are more mouse studies because it is mostly carried on neuron progenitor cells to identify the effect of a virus in the development of...

11 January 2021 5,628 3 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

I need updated CTE Sports related research?

I am looking for epidemiologyCTE for 2019 or 2020. also looking for treatment/management studies studies identifying tau pathology on postmortem studies. I have a few but struggling to find more...

28 October 2020 624 3 View

What PCR and real-time PCR machines perform best?

Hi everyone, I am equipping a new molecular biology/microbiology laboratory and I need to purchase a PCR and/or a real-time PCR machine. So I'm looking for suggestions. Is there a new model that...

11 October 2020 9,791 17 View

What the best way to improve Illumina genomic assembly with nanopore metagenomic data?

I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...

14 July 2020 6,516 3 View

Which phenomenological mathematical models describing the dynamic behaviour of microorganisms in the same ecosystem seem to be the most widely used ?

Hi, As part of a year-round trinomial project, I am looking for information about phenomenological mathematical models describing the dynamic behaviour of several microorganisms in the same...

01 April 2020 9,365 2 View

How do I describe a SDS PAGE when i am specifically looking for p53 protein?

Do I look at the most prominent bands on the figure? or do I look at bands at level 53kDa, even though they are not the most prominent in the image? Would i also mention that multiple bands...

23 March 2020 2,445 3 View

Flow cytometry: How to apply compensation to environmental samples when using an e.coli antibody?

We are using flow cytometry to run e.coli antibodies in environmental water samples. What is the best way to apply compensation? Thanks!

10 March 2020 8,379 4 View

Model protein that I can use as replacement for antibodies ?

Buying antibodies for lab tests is expensive, therefore I would like to know which protein model I could use instead. Thank you in advance.

23 January 2020 4,077 4 View

In the Base/RNAscope protocol, can I replace the commercial Wash Buffer with SSC or similar?

I use BaseScope 2.5 HD Red quite routinely and I found myself using about 3 liter (1 bottle) of Wash Buffer at every run. Although it is the cheapest component of the kit, it's still quite...

12 November 2019 5,759 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

How to measure acetic anhydride solution with 18M sulfuric acid?

I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...

14 February 2021 8,699 3 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View