Can proximal and distal tubules be distinguished with relative certainty in immunofluorescence?
Three possible markers for this purpose:
Anti-Megalin or Anti-PROM1: Both will mark the brush border of proximal tubules
Anti-PROM2: Specific for distal convoluted tubules and collecting ducts
How do you effectively choose a cell line for murine xenografts? What information is needed to effectively compare several cell lines? Is there a way to predict what cell lines will produce...
17 October 2022 3,577 3 View
Most immunofluorescence protocols I've seen apply DAPI as the last step in staining, following the application of primary and secondary antibodies. If DAPI was added first, could it interfere...
13 October 2022 8,091 2 View
A common negative control setup in immunofluorescence is the omission of primary antibodies. What purpose does a negative control serve in these contexts? Is it just to get an idea of the...
19 April 2022 4,037 3 View
Hello I am looking for a suitable adipocyte cell line to treat with human sera. It seems that human adipocyte cell lines are rarer than those of mice (3T3-L1). Is there an advantage to a human...
17 February 2022 3,335 3 View
How does autofluorescence factor into antibody selection in immunofluorescent microscopy? How do you distinguish it visually?
12 January 2022 1,898 3 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.
14 July 2024 485 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
Immunofluorescence staining of mouse spleen paraffin sections showed strong fluorescence signals in red blood cells in the control section without antibodies. Is it autofluorescence of red blood...
02 July 2024 827 3 View
I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...
28 June 2024 353 1 View
I did a frozen section of mouse brain tissue with immunofluorescence staining. My partner and I did c-fos staining together. We used different primary and secondary antibodies for different...
20 June 2024 8,989 1 View
Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...
17 June 2024 3,434 5 View
Could the tissue quality/age be an issue ??
15 June 2024 7,763 0 View
Hello, could you please give me precise details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?
13 June 2024 3,591 1 View