Can proximal and distal tubules be distinguished with relative certainty in immunofluorescence?
Three possible markers for this purpose:
Anti-Megalin or Anti-PROM1: Both will mark the brush border of proximal tubules
Anti-PROM2: Specific for distal convoluted tubules and collecting ducts
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
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11 February 2021 562 6 View
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26 January 2021 866 1 View
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10 January 2021 4,002 5 View